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. 2011 Nov 21;121(12):4820–4837. doi: 10.1172/JCI45677

Figure 6. Overexpression of full-length rNf1, but not Y1587Δ mutant, rescues the dendritic spine phenotype of Nf1+/– neurons in culture.

Figure 6

Mouse cultured cortical neurons were transfected at DIV7 (A) or DIV12 (D) and harvested for staining using neurofibromin and GFP antibodies at DIV10 (A) or DIV18 (D). For WT neurons, GFP-actin and vector control were transfected. For Nf1+/– neurons, GFP-actin was cotransfected with full-length rNf1, full-length Y1587Δ mutant, or vector control, as indicated. B and C, and E and F are quantitative analyses of A and D, respectively. Values in B and E represent the mean plus SEM. The numbers of analyzed dendrites for each group were: (B and C) WT, 79; Nf1+/– control, 106; Nf1+/– rNf1, 37; Nf1+/– Y1587Δ, 59; (E and F) WT, 94; Nf1+/– control, 110; Nf1+/– rNf1, 49; Nf1+/– Y1587Δ, 44. Scale bars: 10 μm in the whole images; 2 μm in the enlarged images (A); 2 μm (D). In C, P < 0.01, Nf1+/– rNf1 versus Nf1+/– control; P < 0.001, Nf1+/– control versus WT and Nf1+/– rNf1 versus Nf1+/– Y1587Δ. In F, P < 0.001, Nf1+/– control versus WT and versus Nf1+/– rNf1, and Nf1+/– Y1587Δ versus Nf1+/– rNf1. **P < 0.01, ***P < 0.001.