Figure 2. Molecular analysis of Notch and NF-κB target gene expression in Kras^G12DTnfa^ΔPdx and Kras^G12DIkk2^ΔPdx pancreases.

(A) Relative mRNA expression of Hes1, Hey1, Batf, Vegf, Igfr1, Myc, and tenascin C in Kras^G12DTnfa^ΔPdx and Kras^G12DIkk2^ΔPdx 3-month PanIN-bearing pancreases was measured by real-time PCR. Data are shown as mean + SD; n = 6. *P < 0.05, **P < 0.01. The experiment was done in duplicate. (B) Immuno­fluorescence staining for Hes1 and E-cadherin in PanIN-bearing pancreases from Kras^G12DTnfa^ΔPdx, Kras^G12DIkk2^ΔPdx, and Kras^G12D mice at 3 months of age. Original magnification, ×40. Blue, DAPI; red, E-cadherin; green, Hes1.