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. 2011 Nov 1;121(12):4787–4795. doi: 10.1172/JCI59150

Figure 1. Murine intestinal LP macrophages express CX3CR1.

Figure 1

(A) Flow cytometry of small (SI) and large (LI) intestine LP cells from C57BL/6 mice, stained for CD45, I-Ab, CD11b, CD11c, F4/80, and CD103. Populations were defined as R1, CD11b DCs; R3, CD11c macrophages; R4, CD11b+ DCs; and R5, CD11c+ macrophages. (B) GFP expression in LP macrophages (MΦ; solid histograms) and DCs (open histograms) in the small and large intestine of Cx3cr1gfp/gfp mice, assessed by flow cytometry and gated as in A. (C) qRT-PCR for Cx3cr1 mRNA expression in isolated intestinal LP APCs from Cx3cr1+/+ mice. Data are representative of 3 (B and C) or more than 3 (A) independent experiments. Error bars represent SD. *P < 0.05 versus respective controls.