Figure 5. Hepatocyte fate tracing after acute CCl4 intoxication.

(A) Adult R26R-EYFP mice were injected with 4 × 10¹¹ viral genomes of AAV8-Ttr-Cre and received a single dose of CCl4 7 days later. Livers were analyzed 2 days after CCl4 intoxication. (B) H&E staining shows pericentral hepatocyte necrosis. (C) Coimmunostaining for EYFP (red) and CK19 (green) confirms hepatocyte loss in pericentral areas, while periportal areas, including bile ducts, appear normal. (D) Coimmunostaining for EYFP (red) and MUP (green) fails to detect EYFP-negative hepatocytes emerging in periportal areas. Pericentral necrotic hepatocytes appear weakly MUP positive (Supplemental Figure 5C). Nuclei were stained with DAPI (blue). Original magnification, ×100, insets ×200. 20 liver sections from 4 mice were analyzed.