Figure 6. Hepatocyte fate tracing after chronic CCl4 intoxication.

(A) Adult R26R-EYFP mice were injected with 4 × 10¹¹ viral genomes of AAV8-Ttr-Cre and, starting 1 week later, received biweekly doses of CCl4 for 6 weeks. Livers were analyzed 3 days after the last CCl4 dose. (B) H&E staining shows liver remodeling, including bridging between periportal areas. (C) Coimmunostaining for EYFP (red) and CK19 (green) shows a cluster of EYFP-negative cells resembling hepatocytes in close proximity to a CK19-positive cell (white arrow). (D) Coimmunostaining for EYFP (red) and MUP (green) confirms the presence of clusters of EYFP-negative, MUP-positive hepatocytes (white arrows). Nuclei were stained with DAPI (blue). Original magnification, ×100, insets ×200. 15 liver sections from 3 mice were analyzed.