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(A) Adult R26R-EYFP mice were injected with 4 × 1011 viral genomes of AAV8-Ttr-Cre and underwent BDL 7 days later. Livers were analyzed 10 days after BDL. (B) H&E staining shows extensive ductular reactions. (C) Coimmunostaining for EYFP (red) and CK19 (green) shows no double-positive cells. Nuclei were stained with DAPI (blue). Original magnification, ×100, insets ×200. 25 liver sections from 5 mice were analyzed.
