Skip to main content
. 2011 Nov 21;121(12):4712–4721. doi: 10.1172/JCI60417

Figure 1. Effects of gefitinib on human and canine corticotroph adenoma cell cultures.

Figure 1

(A) After trans-sphenoidal surgical resection of human ACTH-secreting adenomas, tumor cells were dispersed, cultured, and treated with gefitinib (0.1–10 μM) for 24 hours, and human POMC expression levels were measured by real-time PCR. A representative experiment is shown (normalized by GAPDH). Each bar depicts 5 control replicates and 3 for each dose (0.1–10 μM). (B–D) After trans-sphenoidal surgical resection of canine ACTH-secreting adenomas, tumor cells were dispersed, cultured, and treated with gefitinib (0.1–10 μM) for 24 hours. (B) Canine POMC expression levels were measured by real-time PCR. A representative experiment is shown (normalized by RPS5). (C) ACTH levels in culture media were measured using RIA. (D) Taqman PCR was performed using a specific canine EGFR probe and normalized by canine β-ACTIN. Values are mean ± SEM. *P < 0.05, #P < 0.01.