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. 2011 Nov 7;8(1):15–29. doi: 10.7150/ijbs.8.15

Figure 8.

Figure 8

Effect on target proteins. Semi-quantitative immunochemistry was used to investigate the compound's effect on expression of potential Wnt pathway target genes. CCLP-1 cells were treated with either 10 µM DMAT, 20 µM FH535, 50 µM myricetin, 50 µM quercetin or 10 µM TBB in serum-free DMEM for 5 or 24 hrs. Afterwards, cell blocks were prepared, fixed in formalin and subjected to standard immunochemistry. Staining intensity was used for discrimination of positive versus negative cells. Significant (p<0.05) and highly significant (p<0.01) differences between percentages of positive cells within treated samples and the untreated control for each time point are marked with *, † and **, †† respectively (univariate ANOVA, LSD post-hoc test).