Table 1.
TAP1 primer sequences used in this study.
| Gene name | Primer name | Primer sequences(5'-3') | Primer location | PCR(Tm)( °C) | Size(bp) |
|---|---|---|---|---|---|
| SNP-F | tcggaaatgtggataagagca | intron1 | 56 | 790 | |
| SNP-R | aaacagacggataatgaaagagg | intron3 | |||
| TAP1 | map-F | acctggctcattgctcgtc | intron3 | 61 | 329 |
| map-R | tctgggatgagggtcagtgtag | intron4 | |||
| exp-F | ccagatccagttcaccgaagc | exon6 | 61 | 190 | |
| exp-R | cgggtaggcaaaggagacatt | exon7 | |||
| pEGFP-F | ccgctagccgacctcgtacgccaatg | 5'UTR | 60 | 2274 | |
| pEGFP-R | ccggatcccttaactcaggagcatct | 3'UTR | |||
| β-actin | Actb-F | ggacttcgagcaggagatgg | 61 | 130 | |
| Actb-R | gcaccgtgttggcgtagagg | ||||