One passage expansion on ECM resulted in significant changes of immunophenotype profiles of human bone marrow stromal cells (hBMSCs) in percentage (A) and MFI (B). The measured surface phenotypes consisted of integrin markers (integrins α1 [CD49a], α2 [CD49b], α3 [CD49c], α4 [CD49d], α5 [CD49e], α6 [CD49f], β1 [CD29], β3 [CD61], β4 [CD104], and β5), adhesion molecules (CD24, CD106, CD166), mesenchymal stem cell markers (CD44, CD71, CD73, CD90, CD105), and hematopoietic stem cell markers (CD14, CD45).