FIG. 6.

ECM-expanded hBMSCs were also evaluated for their osteogenic and adipogenic capacities. After incubation in osteogenic medium (OM) or growth medium (GM) for 14 days, osteogenesis was assessed with accumulated calcium deposition stained with Alizarin Red S (ARS) (A) and alkaline phosphatase (ALP) (B) staining and activity assay. After incubation in adipogenic medium (AM) or growth medium (GM) for 14 days, adipogenesis was assessed with intracellular lipid-filled droplet formation stained with ORO and quantification (C). Significant differences were indicated as follows: ***p<0.001. Data were shown as average±SD for n=3. Color images available online at www.liebertonline.com/tea