Figure 4.

Subcellular Locations of Candidate PATs.

C-terminal GFP fusions were transiently expressed in N. benthamiana protoplasts and visualized with confocal microscopy. SGN-U567172 was localized to chloroplasts (top). The same protein without the predicted transit peptide accumulated in the cytoplasm (middle). SGN-U567390 and SGN-U585067 were also located in the cytoplasm, consistent with the lack of identifiable signal peptides. Chloroplasts were visualized by autofluorescence. Chl, chlorophyll.