Table 6.
Untargeted LC-MS Analysis of Tomato Fruit Extracts
| Change in Ph ODO1Lines | Putative ID | M82 | 7817 | 7948 | 7949 | t Test 7817 | t Test 7948 | t Test 7949 |
| n = 5 | n = 9 | n = 9 | n = 3 | |||||
| UP | Feruloyl-dihexoside-deoxyhexoside | 52 | 150 | 993 | 320 | 0.088671 | 0.000005 | 0.000007 |
| UP | 3-O-Feruloylquinic acid | 31 | 529 | 310 | 293 | 0.000001 | 0.000014 | 0.000000 |
| UP | Ferulic acid-hexose I | 229 | 904 | 623 | 806 | 0.000089 | 0.019063 | 0.000016 |
| UP | Ferulic acid-hexose II | 305 | 1,828 | 1,512 | 2,332 | 0.000654 | 0.001690 | 0.000003 |
| UP | 2-(3,4-Dihydroxyphenyl)ethanol; 3′-Me ether, hexoside deoxyhexoside | 38 | 383 | 1,620 | 163 | 0.049449 | 0.000110 | 0.000001 |
| UP | 3-(3,4,5-Trihydroxyphenyl)-1,2-propanediol; 3′,4′-Di-Me ether, hexoside | 33 | 1,141 | 2,090 | 1,436 | 0.000124 | 0.000000 | 0.000003 |
| UP | 3-(3,4,5-Trihydroxyphenyl)-2-propen-1-ol, 3′-Me ether, hexoside | 216 | 1,383 | 768 | 1,036 | 0.000015 | 0.000682 | 0.000001 |
| UP | Acetoxy-tomatine + FA | 1,550 | 23,046 | 3,438 | 2,430 | 0.000791 | 0.004964 | 0.023984 |
| UP | α-Tomatin + FA | 3,575 | 8,670 | 5,333 | 4,432 | 0.076583 | 0.060116 | 0.438996 |
| UP | Benzyl alcohol-hexose-pentose | 39 | 965 | 422 | 774 | 0.000137 | 0.002707 | 0.000177 |
| UP | β-Tomatine | 40 | 1,196 | 1,239 | 1,255 | 0.000023 | 0.000209 | 0.000000 |
| UP | C10H12O3-glutathione ester | 35 | 1,542 | 1,961 | 2,474 | 0.000178 | 0.000086 | 0.000000 |
| UP | Coniferyl aldehyde-C7H12O7 ester | 29 | 4,885 | 8,190 | 6,007 | 0.000020 | 0.000000 | 0.000000 |
| UP | Dihydroconiferyl alcohol-hexoside | 51 | 3,458 | 4,914 | 3,183 | 0.000001 | 0.000000 | 0.000000 |
| UP | Glycoalkaloid | 1,437 | 3,508 | 1,011 | 1,205 | 0.022306 | 0.017561 | 0.338359 |
| UP | Kaempferol-(coumaroyl)dihexose-deoxyhexose | 30 | 211 | 150 | 114 | 0.001640 | 0.002464 | 0.000981 |
| UP | Lycoperoside H or hydroxytomatine II + FA | 588 | 1,507 | 776 | 695 | 0.014544 | 0.027100 | 0.077155 |
| UP | Quercetin-dihexose-deoxyhexose | 654 | 2,577 | 1,729 | 1,683 | 0.000050 | 0.000266 | 0.001391 |
| UNCHANGED | UDP-glucose | 9,128 | 8,675 | 10,927 | 9,510 | 0.819235 | 0.119371 | 0.804161 |
| DOWN | 3-Caffeoylquinic acid (chlorogenic acid) | 1,682 | 1,150 | 350 | 433 | 0.036787 | 0.000001 | 0.001936 |
| DOWN | 4-Caffeoylquinic acid | 369 | 298 | 149 | 180 | 0.217157 | 0.000061 | 0.034939 |
| DOWN | 5-Caffeoylquinic acid | 4,986 | 5,664 | 1,491 | 1,196 | 0.696645 | 0.000005 | 0.002737 |
| DOWN | Tricaffeoyl quinic acid I | 1,981 | 962 | 240 | 575 | 0.000432 | 0.000002 | 0.004658 |
| DOWN | Tricaffeoyl quinic acid II | 1,934 | 1,094 | 165 | 316 | 0.025902 | 0.000000 | 0.002046 |
| DOWN | Dicaffeoylquinic acid II | 654 | 396 | 52 | 104 | 0.013309 | 0.000000 | 0.000601 |
| DOWN | Dicaffeoylquinic acid III | 2,624 | 1,600 | 385 | 458 | 0.055148 | 0.000002 | 0.003025 |
| DOWN | Caffeic acid-hexose III | 327 | 180 | 103 | 222 | 0.023263 | 0.000088 | 0.152436 |
| DOWN | Coumaric acid-hexose I | 321 | 235 | 95 | 604 | 0.679422 | 0.000903 | 0.005196 |
| DOWN | DehydroesculeosideA + FA | 1,854 | 955 | 953 | 966 | 0.000669 | 0.000201 | 0.017773 |
| DOWN | EsculeosideA+ FA | 704 | 392 | 412 | 344 | 0.000811 | 0.000740 | 0.010312 |
| DOWN | Glycoalkaloid | 260 | 29 | 49 | 74 | 0.000000 | 0.000001 | 0.001894 |
| DOWN | Hydroxyphenylethanol-rutinoside | 394 | 115 | 79 | 151 | 0.000176 | 0.000086 | 0.044684 |
| DOWN | Kaempferol-3-O-rutinoside | 711 | 660 | 158 | 63 | 0.693256 | 0.000037 | 0.001177 |
| DOWN | Kaempferol-hexose-deoxyhexose, -pentose | 649 | 523 | 126 | 41 | 0.369613 | 0.000009 | 0.001515 |
| DOWN | Lycoperoside F or G I (+ FA) | 2,819 | 1,350 | 1,593 | 1,547 | 0.000059 | 0.000187 | 0.012161 |
| DOWN | Lycoperoside F or G II + FA | 26,392 | 23,220 | 23,937 | 23,781 | 0.012049 | 0.003229 | 0.021076 |
| DOWN | Naringenin | 1,077 | 258 | 61 | 81 | 0.000035 | 0.000000 | 0.001677 |
| DOWN | Naringenin chalcone | 9,742 | 2,776 | 821 | 1,346 | 0.000001 | 0.000000 | 0.000026 |
| DOWN | Naringenin-chalcone glucoside | 214 | 57 | 40 | 46 | 0.000008 | 0.000000 | 0.001140 |
| DOWN | Phloretin-dihexoside | 732 | 245 | 76 | 22 | 0.000169 | 0.000000 | 0.000112 |
| DOWN | Quercetin-3-O-rutinoside | 26,110 | 23,176 | 5,140 | 5,954 | 0.253163 | 0.000000 | 0.000179 |
| DOWN | Quercetin-hexose-deoxyhexose, -pentose | 8,941 | 6,747 | 582 | 178 | 0.410582 | 0.000823 | 0.041050 |
Peak height (ion count) of (putatively) identified compounds in M82 and ODO1 transgenic fruits. Values are average of the indicated number of fruits harvested at breaker plus 7 d. The amount of all compounds differ significantly (P < 0.05) from wild-type M82 in at least two out of three transgenic lines as indicated in the status row: UP, upregulated; DOWN, downregulated in transgenic lines compared to M82. One unaltered metabolite is labeled “UNCHANGED.”