Figure 8.

The frd3 Mutation Acts Gametophytically to Alter Pollen Development.

(A) to (I) Histological sections of flowers from wild-type FRD3/FRD3 ([A] to [C]), heterozygous FRD3/frd3-7 ([D] to [F]), or homozygous mutant frd3-7/frd3-7 plants ([G] and [H]) stained with periodic acid-Schiff-naphthol blue-black ([A], [B], [D], [E], [G], and [H]) or with Perls/DAB ([C], [F], and [I]).

(A), (D), and (G) Immature anther containing pollen at the two-cell stage.

(B), (C), (E), (F), (H), and (I) Mature anther containing trinucleated pollen before dehiscence. Arrowhead, normal pollen; asterisk, abnormal pollen. Scale bars = 50 μm.