Figure 3.

CBF Transcript Levels Are Not Cold Inducible in Seeds.

The transcript response of CBF1 (A) and the CBF target COR15b (B) in response to sudden cold shock of 4°C in seedlings, imbibed seeds, or during seed maturation, measured by real-time RT-PCR. CBF1 expression levels are induced by cold over 4 levels of magnitude in seedlings but not in seeds. A similar response for COR15b suggests that our analysis of CBF1 is representative of all three CBFs. Data represent the mean and se of two biological replicates per treatment.