Figure 4.

CBFs Are Required for Primary Seed Dormancy and for the Normal Cold-Responsive Gene Expression Required for Dormancy.

(A) The primary dormancy and response to cold stratification of seeds of loss-of-function lines for CBFs 1, 2, and 3, set at either 10, 15, or 20°C. Data represent the mean and se of five independent seed lots for each genotype.

(B) RT-PCR analysis of low-temperature-induced gene expression in wild-type Col-0 seeds and cbf loss-of-function lines. Data represent the mean and se of three biological replicates per genotype. Significant differences from the wild type (t test, P < 0.01) are indicated by asterisks.