Table 1.
Genes with Known Roles in the Regulation of Dormancy or Germination and the Effect of Temperature during Seed Maturation on Transcript Levels in Dry Seeds
| Gene Name | Locus | Average Expression at 20°C | Average Expression at 10°C | Fold Change 10°C/20°C | Q Value (%) |
| Upregulated | |||||
| DOG1 | AT5g45830 | 506.6 ± 305.5 | 6,809.8 ± 925.7 | 13.4423 | 0 |
| GA2ox6 | AT1g02400 | 167.4 ± 24.9 | 816.5 ± 277.1 | 4.8781 | 0.3293 |
| NCED4 | AT4g19170 | 1,412.1 ± 3,637 | 3,546.0 ± 638.6 | 2.5111 | 0.3878 |
| Unchanged | |||||
| GA2ox2 | AT1g30040 | 138.1 ± 85.5 | 319.1 ± 113.2 | 2.3094 | 4.3481 |
| GAI | AT1g14920 | 715.1 ± 312.8 | 1,343.1 ± 68.8 | 1.8782 | 5.1642 |
| RGL3 | AT5g17490 | 1,504.2 ± 391.7 | 2,584.3 ± 68.8 | 1.7181 | 5.1642 |
| ABI2 | AT5g57050 | 1,672.9 ± 184.5 | 2,648.4 ± 88.6 | 1.5832 | 4.3481 |
| RGA | AT2g01570 | 154.9 ± 50.8 | 242.3 ± 44.0 | 1.5650 | 12.9371 |
| RGL2 | AT3g03450 | 212.0 ± 49.7 | 207.4 ± 45.4 | 0.9780 | 20.9340 |
| ABA2 | AT1g52340 | 194.4 ± 10.9 | 176.5 ± 44.8 | 0.9079 | 20.9340 |
| SLY1 | AT4g24210 | 711.0 ± 282.8 | 625.9 ± 81.7 | 0.8803 | 20.9340 |
| FLC | At5g10140 | 1,897.3 ± 300.9 | 1,537.28 ± 306.9 | 0.8102 | 11.050 |
| SPT | AT4g36930 | 100.7 ± 24.6 | 78.1 ± 37.3 | 0.7757 | 11.050 |
| GA3 | AT5g25900 | 3,645.7 ± 864.6 | 2,479.0 ± 206.2 | 0.6799 | 4.3481 |
| GA2 | AT1g79460 | 139.3 ± 68.7 | 80.4 ± 15.3 | 0.5774 | 5.1642 |
| ABI5 | AT2g36270 | 10,066.3 ± 1,560.8 | 5,467.0 ± 1,419.2 | 0.5431 | 1.1283 |
| Downregulated | |||||
| ABI1 | AT4g26080 | 2,087.1 ± 147.2 | 1,044.9 ± 205.0 | 0.5007 | 0.6089 |
| ABI8 | AT3g08550 | 267.4 ± 48.8 | 116.1 ± 53.1 | 0.4342 | 0.9796 |
| ABI3 | AT3g24650 | 2,083.2 ± 87.1 | 859.7 ± 215.6 | 0.4127 | 0.6089 |
| PHYB | AT2g18790 | 1,336.2 ± 170.6 | 423.3 ± 191.1 | 0.3168 | 0.6089 |
| ABA1 | AT5g67030 | 7,696.9 ± 1,126.6 | 2,526.3 ± 792.6 | 0.3144 | 0.6089 |
| PHYE | AT4g18130 | 4,028.0 ± 213.9 | 1,083.3 ± 430.3 | 0.2690 | 0.6089 |
| RDO4/HUB1 | AT2g44950 | 821.3 ± 182.8 | 189.4 ± 60.0 | 0.2305 | 0.6089 |
| NIA2 | AT1g37130 | 238.7 ± 142.0 | 55.3 ± 26.5 | 0.1506 | 0.6089 |
| CYP707A2 | AT2g29090 | 2,603.9 ± 1,033.6 | 263.0 ± 14.8 | 0.1010 | 0.6089 |
Based on a standard 1% false discovery rate, significantly up- and downregulated genes are shown at the top and bottom of the table, respectively. In between, selected genes with no significant change in expression are listed. Values represent mean and sd of the three replicate arrays. The Q value indicates the expected frequency of false positives present in a list of differentially expressed genes containing that probe set, and values of 1% or below are considered significant.