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. 2011 Mar 7;2(2):12. doi: 10.1186/scrt53

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Copyright ©2011 McGinley et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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rHIV-pWPT-EF1-a-GFP-W lentiviral vector modification does not affect msc viability or differentiation potential. (A) Evaluation of rHIV-pWPT-EF1-a-GFP-W vector toxicity on rMSC viability by MTT assay. (B) Representative images (magnification 20X) of non-induced control MSCs and MSCs induced towards the adipogenic pathway (i) non-transduced, (ii) rHIV-pWPT-EF1-a-GFP-W transduced (MOI 100) MSCs. (C) Oil red O positive cells per microscope field (number of differentiated cells). (D) Oil red O quantitation. Data are presented as mean ±SD (n = 5) or as representative images of three independent experiments.