Figure 2.

Differentiation capacity of serum-free expanded human MSCs. (a) Day 14 differentiation cultures from cells expanded for eight additional passages in SFM revealed trilineage mesoderm differentiation, as shown by positive (a) Oil Red O staining for adipocytes, (b) Alcian blue staining for chondrocytes, and (c) alkaline phosphatase staining for osteoblasts. (a, c) 10×, (b) 20× objective. (b) Toluidine blue staining of day 21 chondrogenic micromass-differentiation cultures after three passages in (a) serum-free medium or (b) serum-containing medium.