Fig. 6.

ER isoform-specific agonist effects on NO production are concentration-dependent. In DAF-2 loaded BECs increasing concentrations of ERα-specific agonist THC and ERβ-specific agonist DPN (10 pM–100 nM) resulted in progressively increased NO production (measured at 15 min). DPN-induced NO response saturated at 10 nM, whereas THC-induced response was maximal at 100 nM. The effects of DPN and THC were comparable overall. The curves were fitted with a four-parameter sigmoid (R² = 0.992, THC; R² = 0.996, DPN).