TABLE 3.
Individual donor Papp values for ciprofloxacin and prazosin and atenolol movement across excised human jejunum mounted in Ussing chambers
Ciprofloxacin, prazosin, and atenolol permeability across excised sections of human jejunum from pancreatoduodenectomy. Permeability was determined in the apical-to-basal (Pa-b) and basal-to-apical (Pb-a) directions in adjacent tissue segments, giving net permeability (Pnet = Pb-a − Pa-b). Total drug concentrations in donor compartments were 30 μM for ciprofloxacin, 10 μM for prazosin, and 100 μM for atenolol. Ko143 was present at 10 μM. All receiver drug concentrations were determined by HPLC-tandem mass spectrometry. Individual data from three separate human donors.
| Pa-b | Pb-a | Pnet | Efflux Ratio (Pb-a/Pa-b) | |
|---|---|---|---|---|
| cm/s × 10−6 | ||||
| Donor 1 | ||||
| Ciprofloxacin | 10.14 | 20.67 | 10.53 | 2.04 |
| Ciprofloxacin + Ko143 | 11.02 | 7.45 | −3.57 | 0.68 |
| Prazosin | 0.50 | 3.01 | 2.51 | 6.02 |
| Atenolol | 0.24 | 0.40 | 0.16 | 1.68 |
| Donor 2 | ||||
| Ciprofloxacin | 13.07 | 118.25 | 105.18 | 9.05 |
| Ciprofloxacin + Ko143 | 66.07 | 94.42 | 28.35 | 1.43 |
| Prazosin | 1.61 | 2.59 | 0.98 | 1.61 |
| Atenolol | 0.65 | 0.61 | −0.04 | 0.94 |
| Donor 3 | ||||
| Ciprofloxacin | 11.60 | 19.57 | 7.97 | 1.69 |
| Ciprofloxacin + Ko143 | 15.61 | 13.07 | −2.54 | 0.84 |
| Prazosin | 0.55 | 1.85 | 1.3 | 3.36 |
| Atenolol | 0.59 | 0.89 | 0.30 | 1.51 |