TABLE 1.
Study drugs, reference standard provider, and analytical method information
Study drugs: caffeine, 100-mg tablets (GlaxoSmithKline, Uxbridge, Middlesex, UK); bupropion, 150-mg extended release tablets (Watson Pharmaceuticals, Corona, CA); tolbutamide, 500-mg tablets (Mylan, Pittsburgh, PA); and dextromethorphan, 15-mg Robitussin Cough Gels (Wyeth Consumer Healthcare, New York, NY).
| Reference Standards | Supplier Information | Analytical Method | UPLC/MS/MS Detection Parameters: m/z Transition, Cone Voltage, Collision Energy |
|---|---|---|---|
| Caffeine | Cerilliant (Round Rock, TX) | UPLC/MS/MS and HPLC/UV | 195.1 < 137.9, 15, 20 |
| 1-Methyl uric acid | Sigma-Aldrich (St. Louis, MO) | HPLC/UV | N.A. |
| 1,7-Dimethyl uric acid | Sigma-Aldrich | HPLC/UV | N.A. |
| 1-Methyl xanthine | Sigma-Aldrich | HPLC/UV | N.A. |
| AAMU | Sigma-Aldrich | HPLC/UV | N.A. |
| Bupropion | Cerilliant | UPLC/MS/MS | 240.0 < 184.0, 20, 20 |
| 4-OH-BUP | Cerilliant | UPLC/MS/MS | 256.0 < 238.0, 17, 20 |
| Tolbutamide | Sigma-Aldrich | UPLC/MS/MS | 271.1 < 172.0, 25, 10 |
| 4-OH TOLB | Sigma-Aldrich | UPLC/MS/MS | 287.2 < 188.0, 25, 10 |
| Carboxy tolbutamide | Sigma-Aldrich | UPLC/MS/MS | 301.0 < 201.9, 20, 10 |
| Dextromethorphan | Sigma-Aldrich | UPLC/MS/MS | 272.3 < 215.2, 40, 22 |
| Dextrorphan | Sigma-Aldrich | UPLC/MS/MS | 258.2 < 201.2, 45, 22 |
| 3-Methoxy morphinan | Sigma-Aldrich | UPLC/MS/MS | 258.1 < 201.2, 37, 35 |
| Internal standards for quantification | |||
| d4-Midazolam | Cerilliant | UPLC/MS/MS | 330.0 < 295.2, 40, 27 |
| d4-OH midazolam | Cerilliant | UPLC/MS/MS | 346.0 < 328.2, 40, 22 |
| [13C]Caffeine | Toronto Research Chemicals Inc. (North York, ON, Canada) | UPLC/MS/MS | 198.1 < 139.9, 15, 20 |
| Chlorpropamide | Sigma-Aldrich | UPLC/MS/MS | 277.0 < 174.9, 25, 15 |
| 7-β hydroxy propyl theophylline | Sigma-Aldrich | HPLC/UV | N.A. |
N.A., not applicable.