Fig. 5.
GeLC-MRM Examination of isoforms of Bcl-2 family proteins modulated in melphalan resistance. After observing changes in the expression of the most abundant isoforms of Bcl-xL, Mcl-1, Bid, and Bim, the expression levels of the additional isoforms were investigated in the same cell line models. The gel-excision map is shown (A) with expression levels for Bcl-x (B), Mcl-1 (C), Bim (D), and Bid (E) isoforms. Data are presented as ratios between protein expression in naïve and drug-resistant cells with 8226/LR5 versus RPMI-8226 plotted in white and U266/LR6 versus U266 in black.