Table II. Absolute quantification of selected NFκB survival signaling proteins, Bcl-2 family members, and Fanconi Anemia DNA damage repair proteins in naïve and melphalan resistant multiple myeloma cell lines. The expression level of each protein is reported in attomoles per cell. ND values indicate that the protein was not detected in that cell line. Coefficients of variation for each measurement are given (in %). Student's t-tests were used assess the significance of the differences in protein expression between naïve and drug resistant cell lines.

Protein	RPMI-8226		8226/LR5		p value	U266		U266/LR6		p value
	Expression (amol/cell)	CV (%)	Expression (amol/cell)	CV (%)		Expression (amol/cell)	CV (%)	Expression (amol/cell)	CV (%)
NFκB-1 p105	0.064	14	0.027	23	0.034	0.28	4.7	0.048	6.1	2.2E-4
NFκB-1 p50	0.26	18	0.41	15	0.017	0.086	17	0.11	14	0.11
NFκB-2 p100	0.095	6.3	0.028	1.3	0.015	0.053	7.2	0.024	9.4	1.5E-4
NFκB-2 p52	0.029	20	0.076	20	0.034	0.032	12	0.013	7.0	0.015
Rel A, p65	0.065	5.9	0.037	4.9	0.0043	0.057	16	0.079	8.9	0.027
Rel-B	0.038	0.3	0.058	0.75	0.0069	0.050	0.3	0.056	7.8	0.32
Bcl-2	0.71	8.9	0.78	14	0.23	0.40	0.3	0.49	2.1	0.0044
Bcl-xL	0.36	7.4	1.6	5.8	4.2E-5	0.79	13	0.33	3.6	8.9E-7
Mcl-1	0.79	10	0.24	10	3.6E-4	0.36	7.3	0.20	10	9.6E-4
Bfl-1	2.5	4.6	1.0	20	2.4E-5	ND		ND
Bad	0.015	7.9	0.011	7.1	0.016	0.064	4.7	0.025	14	0.0021
Bid	0.34	10	0.041	10	5.8E-7	0.095	2.7	0.023	6.7	2.9E-5
Bim	0.014	17	0.043	8.0	6.9E-4	0.053	9.1	0.069	14	0.08
Bak	0.54	17	0.46	2.2	0.19	0.55	6.0	0.47	9.0	0.07
Bax	3.8	19	4.4	1.5	0.33	2.3	2.6	1.1	7.9	3.0E-4
FANCD2	0.062	7.4	0.12	13	2.1E-4	0.079	9.7	0.078	2.7	0.9
FANCI	0.045	19	0.078	14	0.0086	0.084	4.9	0.078	4.3	0.051