Fig. 8.
NaOCl stress causes methionine auxotrophy that is abolished by methionine addition. Growth phenotypes of the B. subtilis wild type treated with 75 μm NaOCl at an OD500 of 0.4. A, Methionine was added 30 or 60 min after exposure to 75 μm NaOCl stress and the growth was resumed. B, The concentrations of consumed NaOCl of wild-type cells were monitored in the culture supernatants after exposure to 75 μm NaOCl using the FOX assay. The NaOCl concentrations are given as mean values of three independent experiments with error bars. C, Methionine (75 μm) was added after inoculation to the culture at an OD500 of 0,07 and 75 μm NaOCl was added when cells had reached an OD500 of 0.4.