Fig. 1.
Relative proteome expression in PBM from low versus high BMD subjects. Presented are mean protein expression levels in subjects with high BMD and low BMD, respectively. Approximately 500 ng PBM total proteins were analyzed using LC-nano-ESI-MSE method. Herein, only proteins commonly expressed (in ≥10 PBM samples) and detected at <5.0 ng are presented. ANXA2 protein (IPI00418169.3, IPI00455315.4, and IPI00797556.1) were up-regulated in PBM in low BMD subjects. Representative proteins down-regulated in PBM in low BMD subjects include IPI00419585.9 (Peptidyl-prolyl cis-trans isomerase A, PPIA), IPI00374732.4 (similar to peptidyl-prolyl isomerase A-like, PPIAP19), and IPI00887678.1 (similar to peptidyl-prolyl isomerase A-like, LOC654188). As no evidence of differential expression were found in subsequent Western blot analyses using anti-PPIA antibody (Abnova Catalogue No. H00005478-M01), these proteins and the genes encoding them were not analyzed further in this study.