Fig. 2.
Up-regulation of anxa2 protein in subjects in the low versus high BMD group as verified by Western blot. Three bands of ANXA2 protein were imaged on the blot. According to their locations on the blot, the three bands presumably correspond to ANXA2 protein isoforms IPI00418169.3, IPI00455315.4, and IPI00797556.1, as identified by LC-nano-ESI-MSE. Molecular weight of beta-actin is about 41.7 kDa. M: protein marker (Bio-Rad Catalog No. 161–0375) including a protein with molecular weight of about 25 kDa. Proteins were separated on a 10–20% linear gradient polyacrylamide gel (Bio-Rad Catalog No. 161–1124) by electrophoresis. Presented is a representative blot of triplicate experiments.