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. 2011 Dec 1;22(23):4527–4538. doi: 10.1091/mbc.E11-08-0739

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© 2011 DiPetrillo and Smith. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Dynein activity is WT in the absence of C1d. (A) Diagram of the central apparatus and a single doublet microtubule with associated structures. Central pair projections are labeled. (B) Microtubule sliding velocities of axonemes isolated from WT and three independent FAP74ami transformants (1G11, 2D4, 7A4) in low-calcium (black bars) and high-calcium (gray bars) concentrations. Data represent the average of three trials. WT, n = 56; WT + Ca, n = 64; 1G11, n = 90; 1G11 + Ca, n = 63; 2D4, n = 76; 2D4 + Ca, n = 44; 7A4, n = 63; 7A4 + Ca, n = 58. Error bars, ±SEM. Student's t test p > 0.06 for each FAP74ami strain compared with WT in both low- and high-calcium conditions.