Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 1;22(23):4647–4656. doi: 10.1091/mbc.E11-01-0072

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Komatsu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 2: — Optimization of pairs of FPs for distance-dependent FRET biosensors. (A) Scheme of the PKA or ERK activity sensor consisting of YFPs (donor), a FHA1 or WW phosphopeptide binding domain (ligand domain), a 72-Gly linker, a PKA or ERK substrate (sensor domain), CFPs (acceptor), and nuclear export signal (NES). (B) HeLa cells expressing AKAR3 with various pairs of FPs as indicated were stimulated with 1 mM dbcAMP for 10 min. The gain in FRET/CFP is represented with the SD (n > 5). (C) HeLa cells expressing EKAR with various pairs of FPs as indicated were stimulated with 10 ng/ml EGF for 10 min. The gain in FRET/CFP is represented with the SD (n > 5).