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. 2011 Jul 7;119(11):1569–1574. doi: 10.1289/ehp.1003391

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Incidence of apoptotic germ cell death after treatment with B[a]P (0, 0.001, 0.01, or 0.1 mg/kg BW/day) for 90 days. (A) TUNEL staining of testes (original magnification, 100×) and (B) quantitation of TUNEL-positive germ cells in seminiferous tubules. (C) Immunolocalization of active caspase-3 in testes (original magnification: 400×) and (D) quantitation of active caspase-3–positive germ cells in seminiferous tubules. In A and C, arrows indicate TUNEL-positive germ cells and active caspase-3-positive germ cells, respectively; bar = 100 µm. In B and D, values shown are mean ± SD (n = 180 tubules from nine testes). *p < 0.05 compared with control.