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. 2011 Jul 7;119(11):1569–1574. doi: 10.1289/ehp.1003391

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Alterations in sperm number, sperm motility, and qualitative markers of sperm after treatment with B[a]P (0, 0.001, 0.01, or 0.1 mg/kg BW/day) for 90 days. CON and B[a]P indicate 0 mg/kg BW (control) and 0.01 mg/kg BW groups, respectively. Change (mean ± SD) in sperm number (A) and sperm motility (B). (C) Changes in acrosomal integrity evaluated by LysoTracker DND-26 fluorescence staining (original magnification, 1,600×). (D and E) Changes in ADAM3 proteins detected by immunocytochemistry (D; original magnification, 1,600×; bar = 5 μm) and (E) Western blotting. (F) Densitometric quantification of ADAM3 protein content in sperm protein extracts (mean ± SD). *p < 0.05, and **p < 0.01 compared with control.