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. 2011 Nov 11;4:490. doi: 10.1186/1756-0500-4-490

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Copyright ©2011 Jensen et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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IF/FISH targeting myogenin (IF) and the (CAG)-repeat (FISH) of transduced DM1 fibroblasts. (A and E) Panels display DAPI counterstain, (B and F) FITC/GFP channel, (C and G) Cy3 channel, (D and H) merge of DAPI/GFP/Cy3. (A-D) Cells transduced with GFP display no change in morphology or in the number of Cy3-foci (red) top panel. (E-H) Cells transduced with myoD display a multinuclear morphology, signs of cell fusion (syncytia), and an increased number of Cy3-foci per nucleus. Primary antibodies target myogenin, secondary antibodies are Alexa Fluor 488-labeled. The probe is a Cy3-labeled (CAG)₁₀DNA oligonucleotide. Original magnification × 630.