Figure 3.

The Th2 cytokine production bias in splenocytes was not significantly altered in the F. hepatica-infected mice in the absence of MyD88 in vitro. Splenocytes collected from C57BL/6 mice were stimulated with media alone, anti-CD3, or F. hepatica antigen (FhAg) for 72 h. The levels of IFN-γ, IL-4, IL-5, IL-10, and IL-13 in the supernate were measured indirectly via ELISA. The statistical significant differences were determined via one-way ANOVA followed by a Kruskal-Wallis test. *p < 0.05. Five mice were tested per group. Results are compiled from three independent experiments.