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The lack of MyD88 has no effect on Ym1 and RELMα mRNA expression of macrophages from the peritoneal cavity. Six weeks after F. hepatica infection of C57BL/6 mice, the peritoneal cavity lavages were collected and macrophages were purified. RNA extraction and real-time RT-PCR were used for detection of RELMα (A) and Ym1 (B). mRNA expression is shown as a percent of the positive control sample and β-actin was used to normalize the experiments.
