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Representative photomicrographs of double immunofluorescence (CD31 and KI67) of the urinary bladder mucosa isolated from mice instilled chronically (4 weekly instillations) of: BCG, VEGF165, and VEGF121. The area of CD31+ blood vessels was used to calculated microvessel density (Figure 13A) and the co-localization between CD31+ endothelial cells and KI67 expression was used to calculate the degree of angiogenesis (Figure 13 B).
