Table 2. Mapping the binding region of GOLPH2.

		pGADT7-GOLPH2
		III-A	III-B	III	IV	V	III-IV-V
pGBKT7-GOLPH2	III-A	+ +					+ +
	III-B
	III
	IV
	V
	III-IV-V	+ +					+ +

Each of the gal4 binding domain fusion proteins of truncated GOLPH2 were tested for their interaction with the activation domain fusion proteins to truncated GOLPH2. Strong interactions (++) are indicated by growth on SD/-Trp/-Leu/-His/-Ade plates, while weak interactions (+) are indicated by growth on SD/-Trp/-Leu/-Ade plates with 1 mM 3-amino-124-triazole (3-AT).