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. 2011 Nov 29;6(11):e28147. doi: 10.1371/journal.pone.0028147

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Three-dimensional reconstruction of a P21 GFP+ ephrin-A5(-/-) lens reveals a large cluster of aberrant cells underneath anterior epithelial cells (A). Scale bar, 50 µm. Immunostaining of α-SMA (red) in anterior epithelial cells from lens capsule flat mounts of P21 WT (B) and ephrin-A5(-/-) lenses (C). Only ephrin-A5 (-/-) anterior epithelial cells that are abnormally clustered in the fiber cell layer are obviously positive for α-SMA. Scale bar, 20 µm. Three-dimensional reconstruction of the anterior polar region of P14 GFP+ WT (D) and ephrin-A5(-/-) lenses (F). Scale bar, 50 µm. Enlarged views of anterior epithelial cells in D and F are the boxed areas in panels E and G. Scale bar, 20 µm.