Figure 1.

Proteasome inhibitors induced Mcl-1 accumulation in various cancer cells. (A) Mcl-1 but not Bcl-XL or Bcl-2 accumulation was induced by bortezomib. Western blot assay was performed after human colon carcinoma cell lines (DLD1, LOVO, SW620 and HCT116), human ovarian cancer cell SKOV3 and human lung cancer cell H1299 treated with 0.1-5 μM bortezomib; (B) Human breast cancer cell MCF7 was treated with 0.1-5 μM bortezomib. Different cell lines were treated with 0.5-10.0 μM MG132 (C) or 5-50 μM ALLN (D) for 6 h. Data represent one of two independent experiments with similar results.