Table 1. Genetics of FusE class mutants.
| Identified mutations | |||
| Strain | fusA | rplF | Men/Hem genea |
| FusE L6 | |||
| AH380 | WT | −1 nt 158 (FSb) | none |
| AH352 | WT | −1 nt 106 (FS) | none |
| AH377 | WT | +1 nt 23 (FS) | none |
| AH348 | WT | TGA stop nt 244 | none |
| AH370 | WT | TAA stop nt 229 | none |
| AH346 | WT | −1 nt 238 (FS) | none |
| AH333 | WT | TAA stop nt 249 | none |
| AH392 | WT | −1 nt 383 (FS) | none |
| AH379 | WT | TAA stop nt 418 | none |
| AH349 | WT | −1 nt 404 (FS) | none |
| AH357c | WT | TAA stop nt 301 | none |
| FusE L6 Hem | |||
| AH374 | WT | −5 nts 236−240 (FS) | hemA Ala190Glu |
| AH339 | WT | −1 nt 144 (FS) | hemB Gly87Asp |
| AH337 | WT | −1 nt 220 (FS) | hemB Gly87Asp |
| AH384 | WT | −1 nt 221 (FS) | hemH Gly209Asp |
| FusE L6 Men | |||
| AH378 | WT | −1 nt 221 (FS) | menB Arg101Leu |
| AH351 | WT | −4 nts 45–48 (FS) | gerC3 −1 FS nt 919 |
| AH358b | WT | −1 nt 423 (FS) | menB Val58Phe |
| AH360 | WT | ATA Start nt 3 | menA Arg19Ser |
| AH383 | WT | TAA stop nt 139 | menA Gly87Asp |
| AH362 | WT | TAA stop nt 282 | menA Asp81Tyr |
| AH390 | WT | +1 FS nt 84 | menB Arg 53 Cys |
| AH386 | WT | −1 FS nt 90 | menB Gln 204 stop |
a
All genes known to be involved in hemin and menadione biosynthesis were sequenced in the respective groups to identify mutations, see Table S1.
b
Mutation is predicted to cause a ribosomal frameshift (FS) during translation of the mRNA.
c
AH357 and AH358 were also analysed by CGS in comparison with wild-type 8325-4.