Table 2. Growth compensated mutants of FusA SCVs.

Stock	SCV mutation	Compensating mutation	MIC (µg/ml)
	fusA (EF-G)	fusA (EF-G)	KAN	FA	Na
AH001	WT	WT	2	0.064
AH347	Arg659Ser		48	12
AH517	Arg659Ser	Gly653Ala	12	4	2
AH518	Arg659Ser	Val86Ile	12	8
AH519	Arg659Ser	Val86Leu	12	16
AH520	Arg659Ser	Pro635ProPro	12	4
AH521	Arg659Ser	Ala478Val	32	16
AH523	Arg659Ser	Gly653Cys	6	3
AH350	Arg659Cys		12	12
AH524	Arg659Cys	Val86Leu	8	8	2
AH525	Arg659Cys	Val86Ile	8	4	3
AH527	Arg659Cys	Ala217Thr	32	6
AH530	Arg659Cys	Ala376Val	12	16
AH531	Arg659Cys	Met616Ile	12	4
AH364	Asp434Asn		24	256
AH532	Asp434Asn	Val86Ile	12	256	2
AH534	Asp434Asn	Ala376Val	12	256
AH535	Asp434Asn	Ala655Val	6	8
AH366	Pro114His		12	6
AH536	Pro114His	Gly653Val	6	0.25	2
AH537	Reversion	WT	2	0.094
AH538	Pro114His	Val86Ile	8	2	2
AH539	Pro114His	Ser416Tyr	12	24
AH540	Pro114His	Extragenicb	24	8
AH541	Pro114His	Ile408Thr	16	16
AH542	Pro114His	Gly653Ala	4	1.5
AH544	Pro114His	Gly653Cys	4	0.75

^aN, the number of independent isolates with the same genotype and phenotype.

^bNo compensatory mutation was identified within fusA.