Table 3. Growth compensated mutants of a FusA menadione-auxotrophic SCV.
Strain | SCV mutations | Compensating mutation | MIC (µg/ml) | ||
fusA | menE | menE | KAN | FA | |
AH001a | WT | WT | 2 | 0.064 | |
AH334a | Thr436Ile | Trp67Stop | 128 | 48 | |
AH513a | Thr436Ile | Trp67Stop | CT (−60) | 16-34 | 12-16 |
AH514 | Thr436Ile | Trp67Stop | none found. | 16-34 | 12-16 |
AH515 | Thr436Ile | Trp67Stop | CT (−60) | 16-34 | 12-16 |
AH516 | Thr436Ile | Trp67Stop | none found | 16-34 | 12-16 |
AH001 (8325-4), AH334, and AH513 were also analysed by CGS.