Table 4. Selection of growth-compensated mutants from FusE L6 SCVs.

	Mutations in rplF (ribosomal protein L6)
Strain	SCV mutation	Compensating mutation	MIC (µg/mL)		Na
			KAN	FA
AH001 (WT)			2	0.064
AH379	Gln140Stop (CAA→TAA)		8	6
AH592	replaced	TAA→GAA (Glu)	2	0.064	2
AH593	replaced	TAA→TTA (Leu)	3	0.047	2
AH594	replaced	TAA→AAA (Lys)	3	0.064
AH595	replaced	TAA→TAT(Tyr)	3	0.064	3
AH380	−1 T nt 158		8	6
AH597	−1 T nt 158	+T nt 170	3	0.064	2
AH598	−1 T nt 158	+C nt 173	3	0.047
AH600	−1 T nt 158	+T nt 176	2	0.032
AH377	+A nt 23, KKIID→KKNYstop		8	2
AH588b	+A nt 23	−G nt 18 →NKIFD	3	0.064
AH589b	WT	−A nt 23 →KKIFD	3	0.064
AH590b	+A nt 23	−G nt 18 →KKIID WT	2	0.032	2

^aN, the number of independent isolates with the same genotype and phenotype.

^bSequence complexity of AH377 derivatives.

WT: AAG-AAA-ATT-ATT-GAC (KKIID).

AH377: AAG-AAA-AAT-TAT-TGA (KKNYstop).

AH588: AAT-AAA-ATT-TTT-GAC (NKIFD).

AH589: AAG-AAA-ATT-TTT-GAC (KKIFD), and Glu114Leu (GAA→TTA).

AG590: AAA-AAA-ATT-ATT-GAC (KKIID).