Table 5. Selection of growth-compensated mutants from FusE Hemin and Menadione-auxotrophic SCVs.

	Identified mutations
		Mutations in men or hem genes		MIC (µg/ml)
Strain	rplF (L6)a	Original	Intragenic	KAN	FA
AH001 (WT)b				2	0.064
AH339	−1 FSc nt 144	hemB Gly87Asp		256	8
AH559	−1 FS nt 144	replaced	hemB WT	12	4
AH384	−1 FS nt 221	hemH Gly209Asp		128	12
AH497b	−1 FS nt 221	hemH Gly209Asp	NMId	64	8
AH498	−1 FS nt 221	hemH Gly209Asp	NMI	64	12
AH499	−1 FS nt 221	hemH Gly209Asp	NMI	48	12
AH501	−1 FS nt 221	hemH Gly209Asp	NMI	48	8
AH502	−1 FS nt 221	hemH Gly209Asp	NMI	64	8
AH503	−1 FS nt 221	hemH Gly209Asp	NMI	48	6
AH510	−1 FS nt 221	hemH Gly209Asp	NMI	96	6
AH386	−1 FS nt 90	menB Gln204stop		256	12
AH482	−1 FS nt 90	menB Gln204stop	NMI	48	12
AH483	−1 FS nt 90	replaced	menB WT	16	3
AH484	−1 FS nt 90	replaced	menB 204Ser	24	8
AH485	−1 FS nt 90	replaced	menB 204Lys	16	6
AH486	−1 FS nt 90	replaced	menB 204Lys	12	6
AH487	−1 FS nt 90	menB Gln204stop	NMI	24	8
AH488	−1 FS nt 90	replaced	menB WT	24	8
AH362	TAA stop nt 282	menA Asp81Tyr		256	16
AH479	TAA stop nt 282	menA Asp81Tyr	NMI	256	16
AH481	TAA stop nt 282	menA Asp81Tyr	NMI	192	4
AH351	−4 FS nts 45–48	gerC3 −1 FS nt 919		128	12
AH472	−4 FS nts 45–48	gerC3 −1 FS nt 919	NMI	24	8
AH473	−4 FS nts 45–48	gerC3 −1 FS nt 919	NMI	32	8
AH474	−4 FS nts 45–48	gerC3 −1 FS nt 919	NMI	32	6
AH475	−4 FS nts 45–48	gerC3 −1 FS nt 919	NMI	64	12
AH476	−4 FS nts 45–48	gerC3 −1 FS nt 919	NMI	16	6
AH477	−4 FS nts 45–48	gerC3 −1 FS nt 919	NMI	16	16
AH478	−4 FS nts 45–48	gerC3 −1 FS nt 919	gerC T→G at −59	48	16
AH390	+1 FS nt 84	menB Arg53Cys		256	12
AH489	+1 FS nt 84	menB Arg53Cys	NMI	32	6
AH490	+1 FS nt 84	menB Arg53Cys	NMI	48	6
AH491	+1 FS nt 84	menB Arg53Cys	NMI	64	16
AH492	+1 FS nt 84	menB Arg53Cys	menB Val128Ile	24	6

^aNote that the rplF mutations were published previously [18].

^bAH001 (W.T., 8325-4) and AH497 were also analysed by CGS.

^cFS, frameshift mutation.

^dNMI. no mutation identified.