Figure 1. Schematic representation of the FgVEA deletion strategy. (A).

FgVEA and hygromycin resistance cassette (HPH) are denoted by large black and gray arrows, respectively. Annealing sites of PCR primers are indicated with arrows (see supplementary Table 1 for the primer sequences). (B) A 1,162-bp fragment of FgVEA was used as a probe in Southern blot hybridization analysis. Genomic DNA preparations of the wild-type PH-1, the FgVEA deletion mutant ΔFgVeA-9, and the complement strain ΔFgVeA-9C were digested with Pst I.