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Comparison of titin-bound versus palladin-bound Act-EF34 revealed by residual dipolar coupling NMR (RDC) data. (a) Correlation plot of experimentally measured RDCs for Act-EF34 bound to palladin peptide versus back-calculated RDCs based on the NMR structure of Act-EF34 bound to titin Zr7 (PDB code: 1H8B) using REDCAT. (b) The absolute difference between the observed and expected dipolar couplings (ΔRDC) is plotted as a function of amino acid residue for Act-EF34.
