Table 1.
List of mutations
| Background | Mutated residue | Substitutions | Abbreviation |
|---|---|---|---|
| Single mutants | |||
| wt(α7) | C219 | A, D, T | |
| wt(α7) | S223 | A, T, V | |
| wt(α7) | A226 | D, K, L, N, S | |
| wt(α7) | M254 | L | |
| wt(α7) | I281 | D, F, M | |
| wt(α7) | S285 | A, D, I | |
| wt(α7) | V288 | F, I, S | |
| wt(α7) | G463 | F, V, W | |
| Double mutant | |||
| A226S+M254L | SL | ||
| Triple mutants | |||
| A226S+M254L | C219 | A, D, T | |
| A226S+M254L | I281 | D, F, M | SLM |
| A226S+M254L | S285 | A, D, I | |
| A226S+M254L | V288 | F, I, S | SLF |
| A226S+M254L | G463 | F, V, W | |
| Quadruple mutants | |||
| A226S+M254L+V288F | I281 | F, M | SLMF |
| Quintuple mutants | |||
| A226S+M254L+I281M+V288F | S223 | A, M, T, V | TSLMF |
| A226S+M254L+I281M+V288F | L250 | I, M, Y | |
| A226S+M254L+I281M+V288F | V257 | I, M | |
| A226S+M254L+I281M+V288F | F456 | A | |
| A226S+M254L+I281M+V288F | I459 | F, M | |
| A226S+M254L+I281M+V288F | C460 | Y |
Bold indicates individual mutations combined to make the quintuple TSLMF mutant. The abbreviation wt is wild type.