Table 1. Long Term Gene Expression Related to Prenatal THC or Nicotine Exposure in Experimental Animal Models.

Overview of studies examining the effects of exposure to prenatal THC and nicotine, the psychoactive components of cannabis and cigarettes, respectively, on mRNA expression during adolescence (PND35) and in adulthood (PND62-150).

Treatment	Subject	Age at testing	Effects on mRNA expression	Reference
Δ⁹-THC 5 mg/kg Daily oral GD5-PND24	Wistar rat ♂/♀	PND70	♂ Cnr1: ↑ in HIP ♀ Cnr1: no Δ in CTX, CP, NAc, HIP, AMG	(Garcia-Gil et al., 1999)
Δ⁹-THC 0.15 mg/kg Daily i.v., GD5-PND2	Long Evans rat ♂	PND62	♂ Drd2: ↓ in NAc core and shell	(Dinieri et al., 2011)
Δ⁹-THC 0.15 mg/kg Daily i.v. GD5-PND2	Long Evans rat ♂	PND62	♂ Penk: no Δ in CP, ↑ in NAc core, ↑ in NAc shell, ↑ in AMG ♂ Pdyn: no Δ in DS, NAc core, NAc shell	(Spano et al., 2007)
Nicotine 3 mg/kg Oral GD4-GD15	Sprague- Dawley rat ♂	PND35	mRNA expression changes in CAMs ↓ in CP: Actn1^b, Bai3^a, Cdh13^a , Cntn4^a, Cntn5^a Cntn6^a Ctnna1^b Ctnna2^b Ctnnb1^b Ctnnd2^ab Csmd1a, Dscam, Fyn^b, Lphn3^a, Nlgn1, Postn ↑ in NAc: Postn ↓ in NAc: Ptprd^a ↑ in PFC: Postn ↓ in PFC: Dscam Nlgn1 Nrxn3 ↓ in Amy: Ncam1, Pecam1, Sgcz^a	(Cao et al., 2011)
Nicotine 2mg/kg Daily oral GD1-PND14	Sprague- Dawley rat ♂/♀	PND35	nAchR subunit a3, a4, a5, b4 in ↓ VTA nAchR subunit a3 in ↑ NAc	(Chen et al., 2005)
Nicotine 100 mg/l oral GD7-PND28	Wistar rats	PND35	M1 & M2 muscarinic receptor: no Δ PFC, midbrain, HIP, cerebellum, brainstem	(Zhu et al., 1998)
Nicotine 0.1 mg/kg oral PND1-21 and PND8- P21	Sprague- Dawley ♂/♀	PND35	α2, α3, α4, α7, α2 nAchR: no Δ PFC, HIP, striatum, brainstem, thalamus	(Miao et al., 1998)
Nicotine 0.06/ml Oral	Lister hooded rats ♂	PND150	↑Drd5: Striatum TH, NR4A2, DAT1, DRD4: no Δ striatum, PFC	(Schneider et al., 2011)
Nicotine 2.1 mg/day Daily i.v. GD4-PND1	Sprague- Dawley ♂/♀	PND150	♀↑ AT₁R: total brain tissue ♀♂↓ AT₂R: total brain tissue	(Mao et al., 2008)