FIGURE 2.

Significantly greater B cell elimination is seen using CD80 KO versus CD86 KO donor cells. GVHD was induced using unfractionated donor splenocytes containing roughly equal numbers of donor CD8 T cells. BDF1 recipient mice received either 50 × 10⁶ unfractionated WT splenocytes or unfractionated splenocytes from WT, CD80 KO (80), or CD86 KO (86) donors that were normalized for donor CD8 T cell numbers pre-transfer as described in Materials and Methods. 20M designates F1 mice receiving 20 × 10⁶ WT or CD80KO splenocytes (containing 1.8 × 10⁶ CD8 T cells) or 28 × 10⁶ CD86 KO (containing 2.5 × 10⁶ CD8 T cells) splenocytes. 10M designate F1 mice receiving 10⁷ CD80 KO or 14 × 10⁶ (containing 1.2–1.3 3 10⁶ CD8 T cells) unfractionated splenocytes. Recipient F1 spleens were examined at 14 d after donor cell transfer for the following host populations as described in Materials and Methods: B cells (A), CD11c⁺ DCs (B), Cd11b⁺ macrophages (C), CD4 T cells (D), and CD8 T cells (E). Values represent group mean ± SEM (n = 5 for each group). Over three additional experiments comparing CD80 KO to CD86KO and/or CD80/86 KO donor cells have been performed yielding similar results. *p < 0.05; **p < 0.01; ***p < 0.005.