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CD80 KO→F1 mice exhibit significantly greater peak numbers of effector CD8 T cells. The experimental protocol is as outlined for Fig. 3 and results shown for mice receiving 20 × 10 WT or CD80 KO splenocytes at the times indicated. Donor CD8 T cells were examined for expression of perforin (A), CD107a (B), IFN-γ (C), or TNF (D) as described in Materials and Methods. *p < 0.05; **p < 0.01.
