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. Author manuscript; available in PMC: 2011 Nov 30.
Published in final edited form as: Nitric Oxide. 2008 Apr 24;19(2):146–151. doi: 10.1016/j.niox.2008.04.019

Figure 1. S-nitrosylation of FLIP by NO.

Figure 1

Human lung epithelial BEAS-2B cells were transiently transfected with myc-FLIP plasmid and treated 1 day later with FasL (0-100 ng/ml) in the presence or absence of the NO donor SNP (300 μg/ml) or DPTA NONOate (200 μM), or with the NO inhibitor AG (100 μg/ml) or PTIO (100 μM) for 2 h. Cell lysates (60 μg protein) were immunoprecipitated (IP) using myc antibody and analyzed by Western blot (WB) using S-nitrosocysteine antibody. The immunoblot signals were quantified by densitometry and mean data from independent experiments (one of which is shown here) were normalized to the result obtained in non-treated control. Plots are mean ± S.D. (n = 3). *p < 0.05 versus FasL-treated control. (Reproduced from ref. 22 with permission from the Journal of Biological Chemistry).